Contact :
Matthieu Labousse
matthieu.labousse (arobase)
Tel : +33 (0) 1 40 79 45 97

Joshua McGraw
joshua.mcgraw (arobase)
Tel : +33 (0) 1 40 79 59 62


Gulliver seminars take place on Mondays at 11:30 AM in the F304 room, and typically last one hour including questions. The seminars are in English, and the scientific topics are mainly those studied in the laboratory.

Gulliver Seminar, Pierre Ronceray (CINAM)

Lundi 31 janvier 11:30-12:30 - Bibliothèque PCT - F3.04

Elastically limited liquid-liquid phase separation inside cells

Many intracellular bodies have been shown to be membrane-less liquid droplets that form through liquid-liquid phase separation (LLPS), both in the cytoplasm and in the nucleoplasm. In contrast to the archetypal oil-in-water demixing, the intracellular environment puts mechanical constraints to the formation of large droplets. In the cell nucleus, in particular, the elastic response of the chromatin network has been shown to oppose LLPS. Here we theoretically consider three scenarios by which LLPS can occur in such an elastic network : (i) by cavitation of large droplets that exclude the network, (ii) by forming many mesh-size-scale microdroplets in the pores of the network, and (iii) by permeating through the network and including it in large droplets. We propose simple criteria for which scenario is preferred, introducing a phase diagram controlled by the trade-off between elastic modulus, liquid-liquid surface tension, and liquid-network wetting properties. Our theory predicts the possibility of yet-unobserved mesh-size-limited liquid droplets in the cytoplasm and nucleoplasm.


Seminars  (4)

General recommendations for the speakers

The audience is often heterogeneous - because of the wide range of scientific topics covered in the lab - so planning a talk for a broader audience would be preferred. The seminar is in English, and speakers are thus invited to prepare their slides in English.

The seminar starts at 11:30 AM. The speaker is asked to arrive in the lab at least 15 minutes in advance to set up their computer. The talks last typically 45 minutes, and are followed by a discussion time.